研究業績

2024年

原著論文

• "Engineering the Substrate Specificity of (S)-β-Phenylalanine Adenylation Enzyme HitB"
  Wang D; *Miyanaga, A; Chisuga, T; *Kudo, F; *Eguchi, T
  ChemBioChem 25, e202400383 (2024).

  • DOI: 10.1002/cbic.202400383
• "Versatile filamentous fungal host highly-producing heterologous natural products developed by genome editing-mediated engineering of multiple metabolic pathways"
  Saito, N.; Katayama, T.; Minami, A.; Oikawa, H.; Maruyama, J.*
  Commun. Biol. 7, 1263 (2024).

  • DOI: 10.1038/s42003-024-06958-0

  異分野融合：丸山先生（東京大学）
• "Subcellular compartmentalized localization of transmembrane proteins essential for production of fungal cyclic peptide cyclochlorotine"
  Katayama, T.; Jiang, Y.; Ozaki, T.*; Oikawa, H.*; Minami, A.*; Maruyama, J.*
  Biosci. Biotechnol. Biochem. 88, 1279-1288 (2024).

  • DOI: 10.1093/bbb/zbae122

  異分野融合：丸山先生（東京大学）、尾崎先生（東北大学）
• "Bioinformatics-guided reconstitution of biosynthetic machineries of fungal eremophilane sesquiterpenes"
  Sato, Y.; Shi, X.; Ye, Y.; Domon, S.; Takino, J.; Ozaki, T.; Liu, C.; Oikawa, H.*; Minami, A.*
  ACS Chem. Biol. 19, 861-865 (2024).

  • DOI: 10.1021/acschembio.4c00040

  国際共同研究；叶先生（華中科技大学）、劉先生（東北林業大学）
• "The Post-Polyketide Synthase Modification Mechanism in Hitachimycin Biosynthesis"
  *Kudo, F; Tsuboi, K; Ikezaki, M; Nagayama, D; Kawamura, K; Ando, T; Miyanaga, A; *Eguchi, T
  ChemBioChem 25, e202400405 (2024).

  • DOI: 10.1002/cbic.202400405

総説

• "Biosynthesis of Macrolactam Antibiotics with β-Amino Acid Polyketide Starter Units"
  *Kudo, F
  J. Antibiot. 77, 486-198 (2024).

  • DOI: 10.1038/s41429-024-00742-2

2023年

原著論文

• "Total biosynthesis of melleolides from Basidiomycota fungi: Mechanistic analysis of the multi-functional GMC oxidase Mld7"
  Fukaya, M.#; Nagamine, S.#; Ozeki, M.; Matsuyama, T.; Sato, M.; Miyamoto, K.; Kawagishi H.; Watanabe, K.; Ozaki, T.; Uchiyama, M.; Oikawa, H.*; Minami, A.*
  Angew. Chem. Int. Ed. 62, e202308881 (2023).

  • DOI: 10.1002/anie.202308881

  異分野融合：河岸先生（静岡大学）、内山先生（東京大学）
• "A fungal sesquiterpene biosynthesis gene cluster critical for mutualist-pathogen transition in Colletotrichum tofieldiae"
  Hiruma, K.*; Aoki, S.; Takino, J.; Higa, T.; Utami, Y. D.; Shiina, A.; Okamoto, M.; Nakamura, M.; Kawamura, N.; Ohmori, Y.; Sugita, R.; Tanoi, K.; Sato, T.; Oikawa, H.; Minami, A.; Iwasaki, W.; Saijo, Y.
  Nat. Commun. 14, 5228 (2023).

  • DOI: 10.1038/s41467-023-40867-w

  異分野融合：晝間先生（東京大学）
• "Oxidative bicyclic ring system formation involving indole diterpene biosynthesis: Remarkable substrate tolerance of a prenyltransferase and flavoprotein oxidase"
  Liu, Y.; Ozaki, T.; Minami, A.*; Oikawa, H.*
  Tetrahedron Lett. 117, 154374 (2023).

  • DOI: 10.1016/j.tetlet.2023.154374
• "Structural Basis of Amide-Forming Adenylation Enzyme VinM in Vicenistatin Biosynthesis"
  *Miyanaga, A; Nagata, K; Nakajima, J; Chisuga, T; Kudo, F; *Eguchi, T
  ACS Chem. Biol. 18, 11, 2343-2348 (2023).

  • DOI: 10.1021/acschembio.3c00517
• "Structure-Based Analysis of Transient Interactions between Ketosynthase-like Decarboxylase and Acyl Carrier Protein in a Loading Module of Modular Polyketide Synthase"
  Chisuga, T; Murakami, S; *Miyanaga, A; Kudo, F; *Eguchi, T
  ACS Chem. Biol. 18, 6, 1398-1404 (2023).

  • DOI: 10.1021/acschembio.3c00151
• "Unique Initiation and Termination Mechanisms Involved in the Biosynthesis of a Hybrid Polyketide-Nonribosomal Peptide Lyngbyapeptin B Produced by the Marine Cyanobacterium Moorena bouillonii"
  *Kudo, F; Chikuma, T; Nambu, M; Chisuga, T; Sumimoto, S; Iwasaki, A; Suenaga, K; Miyanaga, A; *Eguchi, T
  ACS Chem. Biol. 18, 4, 875-883 (2023).

  • DOI: 10.1021/acschembio.3c00011
• "Acyltransferase Domain Exchange between Two Independent Type I Polyketide Synthases in the Same Producer Strain of Macrolide Antibiotics"
  *Kudo, F; Kishikawa, K; Tsuboi, K; Kido, T; Usui, T; Hashimoto, J; Shin-ya, K; Miyanaga, A; *Eguchi, T
  ChemBioChem 24, e202200670 (2023).

  • DOI: 10.1002/cbic.202200670
• "Structural Basis of Transient Interactions of Acyltransferase VinK with the Loading Acyl Carrier Protein of the Vicenistatin Modular Polyketide Synthase"
  *Miyanaga, A; Kawada, K; Chisuga, T; Kudo, F; *Eguchi, T
  Biochemistry 62, 1, 17-21 (2023).

  • DOI: 10.1021/acs.biochem.2c00645

総説

• "Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Using a Pantetheine-Type Probe"
  Miyanaga, A; Kudo, F; Eguchi, T
  Non-Ribosomal Peptide Biosynthesis and Engineering: Methods and Protocols, 207-217 (2023).

  • DOI: 10.1007/978-1-0716-3214-7_10
• "Recent advances in the biosynthesis of ribosomally synthesized and posttranslationally modified peptides of fungal origin"
  Ozaki, T.; Minami, A.; Oikawa, H.*
  J. Antibiot. 76, 3-13 (2023).

  • DOI: 10.1038/s41429-022-00576-w
• "Biosynthesis of indole diterpenes: Reconstitution approach in heterologous host"
  Ozaki, T.; Minami, A.*; Oikawa, H.*
  Nat. Prod. Rep. 40, 202-213 (2023).

  • DOI: 10.1039/D2NP00031H
• "Controlled chemical construction in nature: Unified biogenesis accounting for synthesis of fungal dimeric anhydrides"
  Minami, A.*
  Bull. Chem. Soc. Jpn. 96, 1216-1223 (2023).

  • DOI: 10.1246/bcsj.20230187

著書・解説

• "A new trend in biosynthetic studies of natural products: The bridge between the amino acid sequence data and the chemical structure"
  Minami, A.
  New Tide of Natural Product Chemistry (2023).

2022年

原著論文

• "Elucidation of late-stage biosynthesis of phomoidride: Proposal of cyclization mechanism affording characteristic 9-membered ring of fungal dimeric anhydride"
  Yamamoto, S.; Matsuyama, T.; Ozaki, T.*; Takino, J.; Sato, H.; Uchiyama, M.*; Minami, A.*; Oikawa, H.*
  J. Am. Chem. Soc. 144, 20998-21004 (2022).

  • DOI: 10.1021/jacs.2c09308

  異分野融合：内山先生（東京大学）、尾崎先生（東北大学）
• "Structural Insight into the Reaction Mechanism of Ketosynthase Like Decarboxylase in a Loading Module of Modular Polyketide Synthases"
  Chisuga, T; Nagai, A; *Miyanaga, A; Goto, E; Kishikawa, K; Kudo, F; *Eguchi, T.
  ACS Chem. Biol. 17, 1, 198-206 (2022).

  • DOI: 10.1021/acschembio.1c00856
• "Mechanism of S-Adenosyl-L-methionine C-Methylation by Cobalamin-dependent Radical S-Adenosyl-L-methionine Methylase in 1-Amino-2-methylcyclopropanecarboxylic Acid Biosynthesis"
  *Kudo, F; Minato, A; Sato, S; Nagano, N; Maruyama, C; Hamano, Y. Hashimoto, J; Kozone, I; Shin-ya, K; *Eguchi, T
  Org. Lett. 24, 49, 8975-8979 (2022).

  • DOI: 10.1021/acs.orglett.2c03555
• "Heterologous expression of a polyketide synthase ACRTS2 in Aspregillus oryzae produces host selective ACR-toxins: Co-production of minor metabolites"
  Kotani, A.; Ozaki, T.*; Takino, J.; Mochizuki, S.; Akimitsu, K.; Minami, A.*; Oikawa, H.*
  Biosci. Biotechnol. Biochem. 86, 287-293 (2022).

  • DOI: 10.1093/bbb/zbab214

  異分野融合：秋光先生（香川大学）
• "Biosynthetic machineries of anthraquionones and bisanthraquinones in Talaromyces islandicus"
  Fukaya, M.; Ozaki, T.; Minami, A.*; Oikawa, H.*
  Biosci. Biotechnol. Biochem. 86, 435-443 (2022).

  • DOI: 10.1093/bbb/zbac009
• "Structure and biosynthesis of ribosomal lipopeptide antibiotic albopeptins"
  Oikawa, H.*; Mizunoue, Y.; Nakamura, T.; Fukushi, E.; Jiang, Y.; Ozaki, T.; Minami, A.; Isono, K.
  Biosci. Biotechnol. Biochem. 86, 717-723 (2022).

  • DOI: 10.1093/bbb/zbac039

  日本農芸化学会欧文誌論文賞受賞

総説

• "Recent Advances in the Structural Analysis of Adenylation Domains in Natural Product Biosynthesis"
  *Miyanaga, A; Kudo, F; Eguchi, T
  Curr. Opin. Chem. Biol. 71, e102212, 2022 (2022).

  • DOI: 10.1016/j.cbpa.2022.102212
• "Characterization of the Cobalamin-dependent Radical S-adenosyl-L-methionine Enzyme C-Methyltransferase Fom3 in Fosfomycin Biosynthesis"
  Sato, S; Kudo, F; *Eguchi, T
  Methods Enzymol. 669, 45_70 (2022).

  • DOI: 10.1016/bs.mie.2021.11.025
• "Biosynthesis of Cyclitols"
  *Kudo, F; *Eguchi, T
  Nat. Prod. Rep. 39, 1622-1642 (2022).

  • DOI: 10.1039/d2np00024e

著書・解説

• "麹菌が切り拓く天然物化学～麹菌を利用した生物活性天然物の生産"
  南 篤志、尾_太郎、及川英秋
  発酵・醸造食品の最前線II（食品） (2022).

2021年

原著論文

• "One-pot Enzymatic Synthesis of 2-Deoxy-scyllo-inosose from D-Glucose and Polyphosphate"
  *Kudo, F; Mori, A; Koide, M; Yajima, R; Takeishi, R; Miyanaga, A; *Eguchi, T
  Biosci. Biotechnol. Biochem. 85, 1, 108_114 (2021).

  • DOI: 10.1093/bbb/zbaa025
• "Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis"
  *Kudo, F; Kitayama, Y; Miyanaga, A; Numakura, M; *Eguchi, T
  ChemBioChem 22, 9, 1668_1675 (2021).

  • DOI: 10.1002/cbic.202000839
• "Mutational Biosynthesis of Hitachimycin Analogs Controlled by the β-Amino Acid-Selective Adenylation Enzyme HitB"
  *Kudo, F; Takahashi, S; Miyanaga, A; Nakazawa, Y; Nishino, K; Hayakawa, Y; Kawamura, K; Ishikawa, F; Tanabe, G; Iwai, N; Nagumo, Y; Usui, T; *Eguchi, T
  ACS Chem. Biol. 16, 3, 539_547 (2021).

  • DOI: 10.1021/acschembio.1c00003
• "Complex Structure of the Acyltransferase VinK and the Carrier Protein VinL with a Pantetheine Cross-linking Probe"
  *Miyanaga, A; Ouchi, R; Kudo, F; *Eguchi, T
  Acta Crystallographica Section F 77, 294-302 (2021).

  • DOI: 10.1107/S2053230X21008761
• "Biochemical and Mutational Analysis of Radical S-Adenosyl-L-Methionine Adenosylhopane Synthase HpnH from Zymomonas mobilis Reveals that the Conserved Residue Cysteine-106 Reduces a Radical Intermediate and Determines the Stereochemistry"
  Sato, S; *Kudo, F; Rohmer, M; *Eguchi, T
  Biochemistry 60, 38, 2865-2874 (2021).

  • DOI: 10.1021/acs.biochem.1c00536
• "Biosynthesis of cyclochlorotine: Identification of the genes involved in oxidative transformations and intramolecular O,N-transacylation"
  Jiang, Y.; Ozaki, T.*; Liu, C.; Igarashi, Y.; Ye, Y.; Tang, S.; Ye, T.; Maruyama, J.; Minami, A.*; Oikawa, H.*
  Org. Lett. 23, 2616-2620 (2021).

  • DOI: 10.1021/acs.orglett.1c00525

  異分野融合：丸山先生（東京大学）
• "Genome-based discovery of enantiomeric pentacyclic sesterterpenes catalyzed by the fungal bifunctional terpene synthase"
  Jiang, L.; Zhang, X.; Sato, Y.; Zhu, G.; Minami, A.; Zhang, W.; Ozaki, T.; Zhu, B.; Wang, Z.; Wang, X.; Lv, K.; Zhang, J.; Wang, Y. -H.; Gao, S. -S.; Liu, C.; Hsiang, T.; Zhang, L.; Oikawa, H.*; Liu, X.*
  Org. Lett 23, 4645-4650 (2021).

  • DOI: 10.1021/acs.orglett.1c01361

  国際共同研究：劉先生（華東理工大学）
• "Biochemistry-guided prediction of absolute configuration of fungal reduced polyketide"
  Takino, J.; Kotani, A.; Ozaki, T.; Peng, W.; Yu, J.; Guo, Y.; Mochizuki, S.; Akimitsu, K.; Hashimoto, M.; Ye, T.*; Minami, A.*; Oikawa, H.*
  Angew. Chem. Int. Ed. 60, 23403-23411 (2021).

  • DOI: 10.1002/anie.202110658

  国際共同研究：叶先生（北京大学深セン大学院）
• "Biosynthetic studies of phomopsins unveil posttranslational installation of dehydroamino acids by UstYa family proteins"
  Sogahata, K.; Ozaki, T.*; Igarashi, Y.; Naganuma, Y.; Liu, C.; Minami, A.*; Oikawa, H.*
  Angew. Chem. Int. Ed. 60, 25729-25734 (2021).

  • DOI: 10.1002/anie.202111076